期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 54, 期 -, 页码 366-374出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2017.11.034
关键词
Dihydrofisetin; Anti-inflammation; NF-kappa B; ROS; HO-1
资金
- Fundamental Research Funds for the Central Universities [52902-0900201562]
- Natural Science Foundation of China [81403160]
- Traditional Chinese Medicine Bureau of Guangdong Province [20161214]
- Shenzhen Innovation of Science and Technology Commission [ZDSYS201506050935272, JCYJ20170412103841386, 20160607142024828]
Dihydrofisetin is a flavanonol derived from some edible wild herbs and traditional Chinese medicines. It has been found to possess many biological activities. However, the anti-inflammatory potential of Dihydrofisetin remains uncharacterized. The aim of the present study was to investigate the anti-inflammatory activity of Dihydrofisetin and its underlying mechanisms. We found that Dihydrofisetin dose-dependently inhibited lipopolysaccharide-induced productions of nitric oxide (NO) and prostaglandin E-2 (PGE(2)) in RAW 264.7 macrophages, probably through suppressing the protein expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). The expressions of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and monocyte chemotactic protein (MCP-1) were also suppressed. We further demonstrated that Dihydrofisetin inhibited the activation of mitogen-activated protein kinases (MAPKs) pathway and phosphorylation of I kappa B-alpha whereas upregulated the expression of heme oxygenase-1 (HO-1). The in vivo carrageenan-induced mice paw edema study also indicated that treatment with 100 mg/kg of Dihydrofisetin could significantly inhibit carrageenan induced paw edema, decrease the levels of TNF-alpha, IL-6 and MDA, and increase the activity of GSH-Px in paw tissues. Taken together, Dihydrofisetin may act as a natural agent for treating inflammatory diseases by targeting MAPK, NF-kappa B and HO-1 pathways.
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