期刊
INTERNATIONAL ENDODONTIC JOURNAL
卷 52, 期 1, 页码 68-76出版社
WILEY
DOI: 10.1111/iej.12983
关键词
odontoblasts; perivascular cells; pulp biology; reactionary dentine; alpha SMA
资金
- National Institute of Health (NIDCR) [R01-DE016689, R90-DE022526]
Aim To examine the contribution of perivascular cells expressing alpha SMA to reactionary dentinogenesis. Methodology An inducible, Cre-loxP in vivo fate-mapping approach was used to examine the contribution of the descendants of cells expressing the alpha SMA-CreERT2 transgene to reactionary dentinogenesis in mice molars. Reactionary dentinogenesis was induced by experimental mild injury to dentine without pulp exposure. The Student's t test was used to determine statistical significance at *P <= 0.05. Results The lineage tracing experiments revealed that mild injury to dentine first led to activation of alpha SMA-tdTomato(+) cells in the entire pulp chamber. The percentage of areas occupied by alpha SMA-tdTomato(+) in injured (7.5 +/- 0.7%) teeth were significantly higher than in teeth without injury (2 +/- 0.5%). After their activation, alpha SMA-tdTomato(+) cells migrated towards the site of injury, gave rise to pulp cells and a few odontoblasts that became integrated into the existing odontoblast layer expressing Col2.3-GFP and Dspp. Conclusion Mild insult to dentine activated perivascular alpha SMA-tdTomato(+) cells giving rise to pulp cells as well as a few odontoblasts that were integrated into the pre-existing odontoblast layer.
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