期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 98, 期 -, 页码 25-33出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2018.04.010
关键词
Mosquito; Insecticide; Malaria Insect vectors; Metabolism; Hematophagy; Reproduction; Protoporphyrin; Oviposition
资金
- Leverhulme Trust
- Conselho Nacional de Densenvolvimento Cientifico e Technologico (CNPq)
- BBSRC [BB/F021933/1] Funding Source: UKRI
- MRC [MR/M009114/1] Funding Source: UKRI
The mosquito Anopheles gambiae is the principal vector for malaria in sub-Saharan Africa. The ability of A. garnbiae to transmit malaria is strictly related to blood feeding and digestion, which releases nutrients for oogenesis, as well as substantial amounts of highly toxic free heme. Heme degradation by heme oxygenase (HO) is a common protective mechanism, and a gene for HO exists in the An. gambiae genome HO (AgHO), although it has yet to be functionally examined. Here, we have cloned and expressed An. gambiae HO (AgHO) in E. colt. Purified recombinant AgHO bound hemin stoichiometrically to form a hemin-enzyme complex similar to other HOs, with a K-D of 3.9 +/- 6 mu M; comparable to mammalian and bacterial HOs, but 7-fold lower than that of Drosophila melanogaster HO. AgHO also degraded hemin to biliverdin and released CO and iron in the presence of NADPH cytochrome P450 oxidoreductase (CPR). Optimal AgHO activity was observed at 27.5 degrees C and pH 7.5. To investigate effects of AgHO inhibition, adult female A. gambiae were fed heme analogues Sn- and Zn-protoporphyrins (SnPP and ZnPP), known to inhibit HO. These led to a dose dependent decrease in oviposition. Cu-protoporphyrin (CuPP), which does not inhibit HO had no effect. These results demonstrate that AgHO is a catalytically active HO and that it may play a key role in egg production in mosquitoes. It also presents a potential target for the development of compounds aimed at sterilising mosquitoes for vector control.
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