期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 93, 期 -, 页码 37-46出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2017.12.002
关键词
Culex quinquefasciatus; BinAB toxin binding receptor; Cqm1; alpha-glucosidase; Amylomaltase
资金
- DAE PhD fellowship
The activated binary toxin (BinAB) from Lysinibacillus sphaericus binds to surface receptor protein (Cqm1) on the midgut cell membrane and kills Culex quinquefasciatus larvae on internalization. Cqm1 is attached to cells via a glycosyl-phosphatidylinositol (GPI) anchor. It has been classified as a member of glycoside hydrolase family 13 of the CAZy database. Here, we report characterization of the ordered domain (residues 23-560) of Cqm1. Gene expressing Cqm1 of BinAB susceptible mosquito was chemically synthesized and the protein was purified using E. coli expression system. Values for the Michaelis-Menten kinetics parameters towards 4-nitrophenyl alpha-D-glucopyranoside (alpha-pNPG) substrate were estimated to be 0.44 mM (Km) and 1.9 s(-1) (kcat). Thin layer chromatography experiments established Cqm1 as alpha-glucosidase competent to cleave alpha-1,4-glycosidic bonds of maltose and maltotriose with high glycosyltransferase activity to form glucose-oligomers. The observed hydrolysis and synthesis of glucose-oligomers is consistent with open and accessible active-site in the structural model. The protein also hydrolyses glycogen and sucrose. These activities suggest that Cqm1 may be involved in carbohydrate metabolism in mosquitoes. Further, toxic BinA component does not inhibit alpha-glucosidase activity of Cqm1, while BinB reduced the activity by nearly 50%. The surface plasmon resonance study reveals strong binding of BinB with Cqm1 (Kd, 9.8 nM). BinA interaction with Cqm1 however, is 1000-fold weaker. Notably the estimated Kd values match well with dissociation constants reported earlier with larvae brush border membrane fractions. The Cqm1 protein forms a stable dimer that is consistent with its apical localization in lipid rafts. Its melting temperature (T-m) as observed by thermofluor-shift assay is 51.5 degrees C and Ca2+ provides structural stability to the protein. (C) 2017 Elsevier Ltd. All rights reserved.
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