期刊
ACS APPLIED MATERIALS & INTERFACES
卷 7, 期 13, 页码 7093-7100出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b00410
关键词
neural electrode; nanostructure; cell-material interaction; nanoporous gold; neuron-astrocyte co-culture; nanotopography; multifunctional biomaterial; gliosis
资金
- UC Lab Fees Research Program Award [12-LR-237197]
- Research Investments in the Sciences & Engineering (RISE) Award
- UC Davis College of Engineering
- National Science Foundation [DGE-1148897]
- National Institute of Health [T32-GM008799]
- CounterACT Program
- National Institutes of Health Office of the Director
- National Institute of Neurological Disorders and Stroke [U54 NS079202]
- National Institute of Environmental Health Sciences [T32 ES007059]
- Superfund Basic Research Program [P42 ES04699]
- U.S. DOE [DE-AC52-07NA27344]
Designing neural interfaces that maintain close physical coupling of neurons to an electrode surface remains a major challenge for both implantable and in vitro neural recording electrode arrays. Typically, low-impedance nanostructured electrode coatings rely on chemical cues from pharmaceuticals or surface-immobilized peptides to suppress glial scar tissue formation over the electrode surface (astrogliosis), which is an obstacle to reliable neuron-electrode coupling. Nanoporous gold (np-Au), produced by an alloy corrosion process, is a promising candidate to reduce astrogliosis solely through topography by taking advantage of its tunable length scale. In the present in vitro study on np-Aus interaction with cortical neuron-glia co-cultures, we demonstrate that the nanostructure of np-Au achieves close physical coupling of neurons by maintaining a high neuron-to-astrocyte surface coverage ratio. Atomic layer deposition-based surface modification was employed to decouple the effect of morphology from surface chemistry. Additionally, length scale effects were systematically studied by controlling the characteristic feature size of np-Au through variations in the dealloying conditions. Our results show that np-Au nanotopography, not surface chemistry, reduces astrocyte surface coverage while maintaining high neuronal coverage and may enhance neuron-electrode coupling through nanostructure-mediated suppression of scar tissue formation.
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