期刊
IMMUNOLOGY LETTERS
卷 200, 期 -, 页码 33-42出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2018.06.004
关键词
Soluble PD-1; Cancer vaccine; MUC1; Survivin; Anti-tumor immunity
类别
资金
- Specialized Research Fund for the National Natural Science Foundation of China [31300765]
- Jilin Province Science and Technology Program [20160519018JH]
- Changchun city science and technology plan project [17YJ002]
- National Science, Technology Major Project of the Ministry of Science and Technology of China [2014ZX09304314-001]
- Jilin province science and technology development plan project [20180201001YY]
Soluble PD-1 (sPD1) can bind with ligands PD-L1/PD-L2 on the surface of dendritic cells (DCs). Therefore, a sPD1 vaccine fused with an immunogen can increase T cell activation against cancer. Here, we constructed a MUC1 and survivin (MS) combination gene tumor vaccine expressing MS fused with soluble PD-1 (sPD1/MS). To investigate whether the sPD1/MS fusion vaccine could enhance tumor-specific immune responses, its immunogenicity and anti-tumor activity were examined after intramuscular immunization in mice. Compared with the MS DNA vaccine, the specific cytolysis rate of the sPD1/MS fusion DNA vaccine was increased from 21.64% to 34.77%. Moreover, the sPD1/MS vaccine increased the tumor suppression rate from 17.18% to 30.96% and prolonged survival from 6.96% to 19.44% in a murine colorectal cancer model. Combining the sPD1/MS vaccine with oxaliplatin improved the tumor suppression rate to 74.71% in the murine colorectal cancer model. The sPD1/MS vaccine could also exert a good anti-tumor effect, increasing the tumor infiltrated CD8(+) T cells by 6.5 fold (from 0.10% to 0.65%) in the murine lung cancer model. In conclusion, the sPD1/MS vaccine showed good immunogenicity and anti-tumor effect by activating lymphocytes effectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据