期刊
IEEE SENSORS JOURNAL
卷 18, 期 5, 页码 1828-1834出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSEN.2018.2789436
关键词
Immunosensor; Troponin T; lateral flow immunoassay
资金
- Irish Goverrnment PRDSP Program
Here we report an electrochemical ELISA approach for cardiac Troponin T (cTnT) determination based on a lateral flow membrane with underlying screen printed electrodes (<100 Omega/cm(2\)). The thick film transducer was modified with the anti-cardiac Troponin T antibody via physisorption and the electrochemical performance of the immunosensor was evaluated using cyclic voltammetry. The capture antibody coated immunoelectrode employed for electrochemical determination of Troponin T antigen used a sandwich assay format with horseradish peroxidase conjugated signaling antibodies held in the track of a lateral flow sensor strip. A simple two step procedure realized signal acquisition within <20 min (total assay time). The lateral flow electrochemical immunosensor response resulted in a calibration curve with linear response (0-700 ng/ml cTnT) with limit of detection of 0.15 ng/ml.
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