期刊
JALA
卷 20, 期 1, 页码 51-55出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/2211068214555414
关键词
phenotypic screening; plate reader; growth curve; multiwell plate assays
资金
- National Institutes of Health [GM088244]
- Coulter Foundation at the University of Virginia
- National Science Foundation
- Summer Research Internship Program at the University of Virginia School of Medicine
We present a miniaturized plate reader for measuring optical density in 96-well plates. Our standalone reader fits in most incubators, environmental chambers, or biological containment suites, allowing users to leverage their existing laboratory infrastructure. The device contains no moving parts, allowing an entire 96-well plate to be read several times per second. We demonstrate how the fast sampling rate allows our reader to detect small changes in optical density, even when the device is placed in a shaking incubator. A wireless communication module allows remote monitoring of multiple devices in real time. These features allow easy assembly of multiple readers to create a scalable, accurate solution for high-throughput phenotypic screening.
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