期刊
HUMAN MOLECULAR GENETICS
卷 27, 期 8, 页码 1411-1420出版社
OXFORD UNIV PRESS
DOI: 10.1093/hmg/ddy051
关键词
-
资金
- Canadian Institute of Health Research (CIHR)
- Fondation Pierre Lavoie
- NIDDK [K08DK093705]
- NHLBI [P01HL032262, DP2OD022716]
- Burroughs Wellcome Fund
- CIHR [123382]
- Doris Duke Charitable Foundation
- National Sciences and Engineering Research Council of Canada [RGPIN-2016-04597]
- Montreal Heart Institute Foundation
- Canada Research Chair Program
In humans, fetal erythropoiesis takes place in the liver whereas adult erythropoiesis occurs in the bone marrow. Fetal and adult erythroid cells are not only produced at different sites, but are also distinguished by their respective transcriptional program. In particular, whereas fetal erythroid cells express c-globin chains to produce fetal hemoglobin (HbF), adult cells express b-globin chains to generate adult hemoglobin. Understanding the transcriptional regulation of the fetal-to-adult hemoglobin switch is clinically important as re-activation of HbF production in adult erythroid cells would represent a promising therapy for the hemoglobin disorders sickle cell disease and b-thalassemia. We used RNA-sequencing to measure global gene and microRNA (miRNA) expression in human erythroblasts derived ex vivo from fetal liver (n = 12 donors) and bone marrow (n = 12 donors) hematopoietic stem/progenitor cells. We identified 7829 transcripts and 402 miRNA that were differentially expressed (false discovery rate <5%). The miRNA expression patterns were replicated in an independent collection of human erythroblasts using a different technology. By combining gene and miRNA expression data, we developed transcriptional networks which show substantial differences between fetal and adult human erythroblasts. Our analyses highlighted the miRNAs at the imprinted 14q32 locus in fetal erythroblasts and the let-7 miRNA family in adult erythroblasts as key regulators of stage-specific erythroid transcriptional programs. Altogether, our results provide a comprehensive resource to prioritize genes that may modify clinical severity in red blood cell (RBC) disorders, or genes that might be implicated in erythropoiesis by genome-wide association studies of RBC traits.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据