期刊
HUMAN IMMUNOLOGY
卷 79, 期 8, 页码 594-601出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.humimm.2018.05.002
关键词
HLA expression; HLA-DQB1; HLA-DQB2; Pre-implantation biopsies; Kidney graft dysfunction
类别
资金
- Associacao Fundo de Incentivo Pesquisa (AFIP), Sao Paulo, Brazil
Background: Accurate pre-transplant prediction of late graft function remains an unmet need in kidney transplantation. The aim of this study was to evaluate HLA genes expression levels in pre-implantation biopsies (PIB) of deceased donor kidneys as markers for long-term graft outcome. Methods: HLA genes expression analysis was initially performed using microarray data of 53 PIB, previously generated by our laboratory. The validation analysis was performed by real-time PCR in 116 PIS from an independent cohort. Results: The microarray data showed association between high expression levels of HLA class II genes, especially HLA-DQB1 and -DQB2, in kidneys from young (18 to 49-year-old) donors and poor (eGFR < 45 mL/min/1.73 m(2)) 1- and 5-year graft function. A subsequent study in an independent cohort, in which only HLA-DQB2 expression was evaluated, validated the association between increased HLA-DQB2 expression in PIB of kidneys from young donors and poor 1-year graft function: expression levels >= 0.0025 relative units conferred an odds ratio of 22.5, with positive and negative predictive values of 71.4% and 90.0%, respectively. Conclusion: Heightened expression of HLA-DQB1 and -DQB2 in PIB are promising tools for pre-transplant risk assessment of poor late graft function in transplants with kidneys from 18 to 49-year-old donors.
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