期刊
HISTOCHEMISTRY AND CELL BIOLOGY
卷 149, 期 3, 页码 209-218出版社
SPRINGER
DOI: 10.1007/s00418-018-1635-3
关键词
Adipocyte hypertrophy; Immunohistochemistry; Image analysis; Macrophages; Frozen tissue
资金
- Canadian Institutes of Health Research (CIHR) [MOP-64182]
- Centre de recherche en endocrinologie moleculaire et genomique humaine (CREMOGH)
- Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Quebec (CRIUCPQ)
- Canadian Institutes of Health Research (CIHR) (Frederick Banting Scholarship)
- Canadian Institutes of Health Research (CIHR) (Charles Best Canada Graduate Scholarship)
- Fonds de la recherche du Quebec-Sante (FRQS)
- FRQS
- CIHR (Banting Postdoctoral Fellowships)
Histomorphometric analyses of adipose tissue usually require formalin fixation of fresh samples. Our objective was to determine if intact, flash-frozen whole adipose tissue samples stored at - 80 A degrees C could be used for measurements developed for fresh-fixed adipose tissues. Portions of adipose tissue samples were either formalin-fixed immediately upon sampling or flash-frozen and stored at - 80 A degrees C and then formalin-fixed during the thawing process. Mean adipocyte diameter was measured. Immunohistochemistry was performed on additional samples to identify macrophage subtypes (M1, CD14 + and M2, CD206 +) and total (CD68 +) number. All slides were counterstained using haematoxylin and eosin (H&E). Visual inspection of H&E-stained adipose tissue slides performed in a blinded fashion showed little or no sign of cell breakage in 74% of frozen-fixed samples and in 68% of fresh-fixed samples (p > 0.5). There was no difference in the distribution frequencies of adipocyte sizes in fresh-fixed vs. frozen-fixed tissues in both depots (p > 0.9). Mean adipocyte size from frozen-fixed samples correlated significantly and positively with adipocyte size from fresh-fixed samples (r = 0.74, p < 0.0001, for both depots). The quality of staining/immunostaining and appearance of tissue architecture were comparable in fresh-fixed vs. frozen-fixed samples. In conclusion, intact flash-frozen adipose tissue samples stored at - 80 A degrees C can be used to perform techniques conventionally applied to fresh-fixed samples. This approach allows for retrospective studies with frozen human adipose tissue samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据