期刊
HELICOBACTER
卷 23, 期 2, 页码 -出版社
WILEY
DOI: 10.1111/hel.12467
关键词
detection; genotypic; Helicobacter pylori; phenotypic; resistance
资金
- National Natural Science Foundation of China [81460115]
- Special Funds of Graduate Student Innovation Project in Jiangxi Province [YC2014-S081]
- National Key Research and Development Program of China [2016YFC1302201]
- Science and Technology Projects of Jiangxi Province [2014BBG70019]
BackgroundAntibiotic susceptibility testing is essential for tailored treatments to cure Helicobacter pylori (H.pylori) infection. However, phenotypic methods have some limitations. ObjectivesTo evaluate the feasibility of genotypic detection methods compared with phenotypic detection methods using samples taken from H.pylori-infected patients. MethodsLiterature searches were conducted in the following databases (from January 2000 to November 2016): PubMed, Embase, the Cochrane Library, and Web of Science. A meta-analysis and systematic review was performed for studies that compared genotypic methods with phenotypic methods for the detection of H.pylori antibiotic susceptibility. ResultsThis meta-analysis showed that the pooled sensitivity, specificity, and diagnostic odds ratio (DOR) for the A2142G/C and/or A2143G combination for the detection of clarithromycin resistance in the strain samples were 0.97 (95% CI: 0.94-0.99), 1.00 (95% CI: 0.99-1.00), and 13742 (95% CI: 1708-110554), respectively. The pooled sensitivity, specificity, and DOR for the A2142G/C and/or A2143G combination for the detection of clarithromycin resistance in biopsy samples were 0.96 (95% CI: 0.90-0.99), 0.96 (95% CI: 0.91-0.99), and 722 (95% CI: 117-4443), respectively. The summarized sensitivity, specificity, and DOR value for the ability of the genotypic methods to detect quinolone resistance in biopsy specimens were 0.97 (95% CI: 0.87-0.99), 0.99 (95% CI: 0.92-1.00), and 6042 (95% CI: 486-75143), respectively. ConclusionThe genotypic detection methods were reliable for the diagnosis of clarithromycin and quinolone resistance in the strain and biopsy specimens. The A2142G/C and/or A2143G combination had the best sensitivity and specificity for the detection of clarithromycin resistance.
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