期刊
HEART LUNG AND CIRCULATION
卷 27, 期 5, 页码 E51-E58出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.hlc.2017.04.011
关键词
LncRNA AK12348; Myocardial IRI; A/R; LDH; PARP
资金
- Program of Education Department of Zhejiang province [Y201223954, Y201534626]
- Program of Science and Technology of Zhejiang province [2015KWB150]
- program of Administration of Traditional Chinese Medicine of Zhejiang Province [2015ZA047]
Backgroud Recently long non-coding RNAs (lncRNAs) have attracted attention in several biomedical fields. The purpose of this study is to investigate the profile of myocardial lncRNAs and their potential roles in myocardial ischaemia-reperfusion injury (IRI). Methods EdgeR bioconductor package was used to screen differentially expressed lncRNAs in myocardial IRI, and lncRNA AK12348 was selected. The mRNA levels of lncRNA AK12348 in normal and anoxia/reoxygenation (A/R) cardiomyocytes were determined by qRT-PCR. After transfection with siRNA-lncRNA, AK12348, LDH release and cell apoptotic rates in normal and A/R cardiomyocytes were determined. The protein expression values of PARP and Caspase-3 were also determined by western blotting. Results The relative level of lncRNA AK12348, LDH release and cell apoptotic rate in A/R cardiomyocytes was significantly higher than that in normal cardiomyocytes. After transfection with siRNA-lncRNA AK12348, LDH release and cell apoptotic rates in A/R cardiomyocytes were reduced, while the values in normal cardiomyocytes had almost no change. The protein expression values of PARP and Caspase-3 in A/R cardiomyocytes were much higher than the Control. After knockdown of lncRNA AK12348, the values decreased. Conclusion Long non-coding RNAs AK12348 could be potential therapeutic targets for the treatment of myocardial IRI.
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