期刊
GYNECOLOGIC ONCOLOGY
卷 148, 期 2, 页码 281-285出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygyno.2017.12.004
关键词
Ovarian carcinoma; Methylation; Homologous recombination; Mutation
资金
- University of Washington School of Medicine Medical Student Research Training Program (MSRTP)
- Stand Up To Cancer - Ovarian Cancer Research Fund Alliance - National Ovarian Cancer Coalition Dream Team Translational Research Grant [SU2C-AACR-DT16-15]
Objective. In ovarian carcinoma, mutations in homologous recombination DNA repair (HRR) genes, including BRCA1 and RAD51C, are associated with increased survival and specific clinical features. Promoter hypermethylation is another mechanism of reducing gene expression. We assessed whether BRCA1 and RAD51C promoter hypermethylation is associated with similar survival and clinical characteristics. Methods. Promoter methylation of BRCA1 and RAD51C was evaluated using methylation-sensitive PCR in 332 primary ovarian carcinomas. Damaging germline and somatic mutations in 16 HRR genes were identified using BROCA sequencing. Results. BRCA1 methylation was detected in 22 carcinomas (6.6%) and RAD51C methylation in 9 carcinomas (2.7%). These small numbers limited the power to detect differences in survival and platinum sensitivity. Mutations in one or more HRR genes were found in 95 carcinomas (29%). Methylation of BRCA1 or RAD51C was mutually exclusive with mutations in these genes (P = 0.001). Patients whose carcinomas had BRCA1 methylation (57.7 years +/- 2.5) or BRCA1 mutations (54.1 years +/- 1.4) were younger than those without (633 years +/- 0.8; P = 0.029, P < 0.0001). BRCA1 methylation and germline BRCA1 mutation were associated with high grade serous (HGS) histology (P = 0.045, P = 0.001). BRCA1 mutations were associated with increased sensitivity to platinum chemotherapy while BRCA1 methylation was not (P = 0.034, P = 0.803). Unlike HRR mutations, methylation was not associated with improved overall survival compared to cases without methylation or mutation. Conclusions. Patients with BRCA1-methylated carcinomas share clinical characteristics with patients with BRCA1-mutated carcinomas including younger age and predominantly HGS histology. However, unlike mutation, RAD51C and BRCA1 methylation were not associated with improved survival or greater sensitivity to platinum chemotherapy. (C) 2017 Elsevier Inc. All rights reserved.
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