期刊
GENE
卷 638, 期 -, 页码 60-65出版社
ELSEVIER
DOI: 10.1016/j.gene.2017.09.061
关键词
MicroRNA; Gene regulation; Begomovirus; Cotton leaf curl disease; RNA interference
资金
- University Grants Commission (Govt of India) [MRP43-478/2014]
- UGC
- ICMR
- SERB-DST
Cotton leaf curl Burewala virus (CLCuBV, genus Begomovirus) causes devastating cotton leaf curl disease. Among various known virus controlling strategies, RNAi-mediated one has shown potential to protect host crop plants. Micro(mi) RNAs, are the endogenous small RNAs and play a key role in plant development and stress resistance. In the present study we have identified cotton (Gossypium hirsutum)-encoded miRNAs targeting the CLCuBV. Based on threshold free energy and maximum complementarity scores of host miRNA-viral mRNA target pairs, a number of potential miRNAs were annotated. Among them, ghr-miR168 was selected as the most potent candidate, capable of targeting several vital genes namely Cl, C3, C4, VI and V2 of CLCuBV genome. In addition, ghr-miR395a and ghr-miR395d were observed to target the overlapping transcripts of Cl and C4 genes. We have verified the efficacy of these miRNA targets against CLCuBV following suppression of RNAi-mediated virus control through translational inhibition or cleavage of viral mRNA.
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