期刊
FUTURE VIROLOGY
卷 13, 期 7, 页码 475-482出版社
FUTURE MEDICINE LTD
DOI: 10.2217/fvl-2018-0010
关键词
adeno-associated virus vectors; anal tumors; CRISPR/Cas; gene therapy; human papillomavirus
类别
资金
- National Cancer Institute [R21-CA198050]
Aim: The goal of this study was to determine if a single AAV vector, encoding Cas9 and guide RNAs specific for the HPV16 E6 and E7 genes, could inhibit the growth of an HPV16-induced tumor in vivo. Materials & methods: We grew HPV16(+), patient-derived anal cancer explants in immunodeficient mice and then challenged these by injection of AAV-based vectors encoding Cas9 and control or HPV16-specific guide RNAs. Results & conclusion: We observed a significant and selective reduction in tumor growth when the HPV16 E6 and E7 genes were targeted using Cas9. These studies provide proof of principle for the hypothesis that CRISPR/Cas has the potential to be used to selectively treat HPV-induced tumors in humans.
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