期刊
FUTURE VIROLOGY
卷 10, 期 2, 页码 169-183出版社
FUTURE MEDICINE LTD
DOI: 10.2217/FVL.14.96
关键词
bioluminescence imaging; BLI; CCD camera; cytomegalovirus; herpes simplex; influenza; luciferase; pathogenesis; viral dissemination; virus imaging
类别
资金
- NIH/NIAID [R21AI090156, RO1AI100933]
Bioluminescence imaging (BLI) has emerged as a powerful tool in the study of animal models of viral disease. BLI enables real-time in vivo study of viral infection, host immune response and the efficacy of intervention strategies. Substrate dependent light emitting luciferase enzyme when incorporated into a virus as a reporter gene enables detection of bioluminescence from infected cells using sensitive charge-coupled device (CCD) camera systems. Advantages of BLI include low background, real-time tracking of infection in the same animal and reduction in the requirement for larger animal numbers. Transgenic luciferase-tagged mice enable the use of pre-existing nontagged viruses in BLI studies. Continued development in luciferase reporter genes, substrates, transgenic animals and imaging systems will greatly enhance future BLI strategies in viral research.
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