期刊
FUNGAL BIOLOGY
卷 122, 期 10, 页码 965-976出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2018.06.001
关键词
Aquatic hyphomycetes taxonomy; Barcode; COX1; D1/D2; ITS
类别
资金
- Czech Collection of Microorganisms
- NSERC Discovery Grant
- FCT [SFRH/BPD/103865/2014, UID/MAR/04292/2013]
The taxonomy of the aquatic hyphomycete genus Articulospora (Ascomycota, Pezizomycotima, Leotiales, Helotiaceae) is based on the morphology of the generative phase of its lifecycle. The type species is Articulospora tetracladia, which is distributed worldwide. Its most frequent populations in nature have dimorphic conidia, differing by the extent of conidial branching (i.e., one or two levels of branching). Some strains, stable in culture, produce exclusively conidia of one type. With the molecular analyses employed here and the relatively low number of available isolates (20), separation based on branching of conidia has not been fully supported. Therefore we propose to retain the broad concept of A. tetracladia with dimorphic conidia. Among the three gene sequences tested as potential barcodes, the internal transcribed spacer (ITS) gene was the most promising region. All strains yielded amplifiable DNA which provided adequate resolution, according to accepted ranges in inter/intraspecific differences, to differentiate among the three Articulospora and two Fontanospora species that were tested (Articulospora atra, Articulospora proliferate, A. tetracladia, Fontanospora eccentrica, Fontanospora fusiramosa). D1/D2 primers also permitted amplification in all strains, however without much resolution. Amplification of the COX1 gene sequence was least consistent. (C) 2018 The Author(s). Published by Elsevier Ltd on behalf of British Mycological Society.
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