期刊
FUNGAL BIOLOGY
卷 122, 期 2-3, 页码 121-130出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2017.11.003
关键词
Arbuscular mycorrhizal fungi; Arsenic detoxification; Arsenite efflux pump; Methyl transferases; Rhizophagus irregularis
类别
资金
- National Council for Science and Technology (CONACyT) of Mexico [246225]
- Institute Politecnico Nacional (IPN) of Mexico [2014-720-1-63]
- Institute Politecnico Nacional of Mexico [SIP 20144103, SIP 20150775]
- Agriculture and Food Research Initiative of the U.S. Dept. of Agriculture, National Institute of Food and Agriculture [2014-67013-21571]
Plants associated with arbuscular mycorrhizal fungi (AMF) increase their tolerance to arsenic-polluted soils. This study aims to investigate the genes involved in the AMF molecular response to arsenic pollution. Genes encoding proteins involved in arsenic metabolism were identified and their expression assessed by PCR or RT-qPCR. The As-inducible gene GiArsA (R. irregularis ABC ATPase component of the ArsAB arsenite efflux pump) and two new genes, an arsenate/arsenite permease component of ArsAB (RiArsB) and a methyltransferase type 11 (RiMT-11) were induced when arsenate was added to two compartment in vitro monoxenic cultures of R. irregularis-transformed carrot roots. RiArsB and RiMT-11 expression in extraradical hyphae in response to arsenate displayed maximum induction 4-6 h after addition of 350 pM arsenate. Their expression was also detected in colonized root tissues grown in pots. or in the root-fungus compartment of two-compartment in vitro systems. We used a Medicago truncatula double mutant (mtpt4/mtpt8) to demonstrate that RiMT-11 and RiArsB transcripts accumulate in response to the addition of arsenate but not in response to phosphate. These results suggest that these genes respond to arsenate addition regardless of non-functional Pi symbiotic transport, and that RiMT-11 may be involved in arsenate detoxification by methylation in AMF-colonized tissues. (C) 2017 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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