期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 128, 期 -, 页码 157-164出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2018.02.002
关键词
miniSOG; KillerRed; SuperNova; Reactive oxygen species; Singlet oxygen; Superoxide; Optogenetics; Photosensitization; Photosensitizer
资金
- National Institutes of Health [R01 NS092558]
- Institutional Ruth L. Kirschstein National Research Service Award [GM068411]
Oxidants play an important role in the cell and are involved in many redox processes. Oxidant concentrations are maintained through coordinated production and removal systems. The dysregulation of oxidant homeostasis is a hallmark of many disease pathologies. The local oxidant microdomain is crucial for the initiation of many redox signaling events; however, methods to control oxidant product are limited. Some fluorescent proteins, including GFP, TagRFP, KillerRed, miniSOG, and their derivatives, generate oxidants in response to light. These genetically -encoded photosensitizers produce singlet oxygen and superoxide upon illumination and offer spatial and temporal control over oxidant production. In this review, we will examine the photosensitization properties of fluorescent proteins and their application to redox biology. Emerging concepts of selective oxidant species production via photosensitization and the impact of light on biological systems are discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据