期刊
FOOD RESEARCH INTERNATIONAL
卷 111, 期 -, 页码 198-204出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2018.05.018
关键词
Acid adaptation; Probiotics; Cranberry; Lemon; Pomegranate; Stress adaptation; cis-11,12-Methylene octadecanoic acid; Cyclopropane fatty acid; RT-PCR; Cyclopropane synthase (cfa); beta-Ketoacyl-acyl carrier protein synthase III (FabH)
Pre-treatment of stationary phase cells of Lactobacillus plantarwn NCMIB 8826 with citric acid (pH 3 to 6) for a short period of time significantly improved subsequent cell survival in several highly acidic fruit juices namely cranberry (pH 2.7), pomegranate (pH 3.5), and lemon & lime juices (pH 2.8). Although the mechanism for this adaptation is still unclear, the analysis of the cellular fatty acid content of acid adapted cells and the reverse transcription polymerase chain reaction (RT-PCR) showed a significant increase (by similar to 1.7 fold) of the cellular cyclopropane fatty acid, cis-11,12-methylene octadecanoic acid (C-19:0cyclow7c) and a significant upregulation (similar to 12 fold) of cyclopropane synthase (cfa) were observed, respectively, during acid adaptation. It is likely that these changes led to a decrease in membrane fluidity and to lower membrane permeability, which prevents the cells from proton influx during storage in these low pH fruit juices.
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