期刊
FOOD RESEARCH INTERNATIONAL
卷 105, 期 -, 页码 537-547出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2017.11.046
关键词
Propolis by-product; ROS and RNS; Antioxidant activity; Caco-2; HT29-MTX
资金
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/Coordination for the Improvement of Higher Education of Brazil) [88881.135492/2016-01-PDSE]
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/National Counsel of Technological and Scientific Development of Brazil)
- FINEP (Financiadora de Estudos e Projetos/Financier of Studies and Projects of Brazil)
- FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE Operational Programme for Competitiveness and Internationalisation (POCI), Portugal
- Portuguese funds through FCT - Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Inovacao [POCI-01-0145-FEDER-007274]
- FCT [PEst-C/EQB/LA0006/2013, NORTE-07-0124-FEDER-000069]
- QREN [NORTE-07-0124-FEDER-000069]
- European Union (FEDER funds through COMPETE)
- FCT, Foundation for Science and Technology [LAQV/UID/QUI/50006/2013]
- [UID/QUI/50006]
Propolis is a natural adhesive resinous compound produced by honeybees to protect hives from bacteria and fungi, being extremely expensive for food industry. During propolis production, a resinous by-product is formed. This resinous waste is currently undervalued and underexploited. Accordingly, in this study the proximate physical and chemical quality, as well as the antioxidant activity, radical scavenging activity and cell viability of this by-product were evaluated and compared with propolis in order to boost new applications in food and pharmaceutical industries. The results revealed that the by-product meets the physical and chemical quality standards expected and showed that the propolis waste contains similar amounts of total phenolic content (TPC) and total flavonoid content (TFC) to propolis. Also, a good scavenging activity against reactive oxygen and nitrogen species (ROS and RNS, respectively) determined by the assays of superoxide anion radical (O-2(center dot-)) hydrogen peroxide (H2O2), hypochlorous acid (HOCl), nitric oxide (center dot NO) and peroxyl radical (ROO center dot) were determined. Linear positive correlations were established between the TPC of both samples and the antioxidant activity evaluated by three different methods (DPPH, ABTS and FRAP assays). The extracts were also screened for cell viability assays in two different intestinal cell lines (HT29-MTX and Caco-2), showing a viability concentration-dependent. Similarly, the Artemia sauna assay, used to assess toxicity, demonstrated the concentration influence on results. Finally, the antifungal activity against ATCC species of Candida was demonstrated. These results suggest that propolis by-product can be used as a new rich source of bioactive compounds for different areas, such as food or pharmaceutical.
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