期刊
FOOD CONTROL
卷 88, 期 -, 页码 139-148出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2017.11.041
关键词
Lactobacillus reuteri R29; Phenyllactic acid; Reuterin; Antifungal; Bread making
资金
- Irish Government under the National Development Plan through the research program FIRM/RSF/CoFoRD
- Irish Department of Agriculture, Food and the Marine
Lactic acid bacteria have shown great potential as bio-preservative agents to maintain high food quality and safety. The strain Lactobacillus reuteri R29 is reported to have a broad spectrum of antifungal activity and suitability for application in food systems. Its antifungal activity is predominantly based on phenyllactic acid (PLA). Furthermore, it is potentially a producer of reuterin, a potent antimicrobial agent. This study focused on increasing the antifungal activity in vitro by supplementation of the growth medium with phenylalanine and glycerol to increase the yield of PLA and reuterin, respectively. For PLA, the addition of 1.5% phenylalanine (w/v) to MRS, resulted in significantly increased accumulation of PIA and antifungal performance against Fusarium culmorum. Supplementation of MRS with 500mM glycerol combined with a reduced glucose content (1.5%) showed the highest reuterin accumulation combined with fungal inhibition. To investigate the antifungal performance in situ, these cell-free supernatants (cfs) were applied in a bread system. The application of PLA-enriched cfs resulted in significantly extended (4 days) microbial shelf life compared to the control. The reuterin-enriched medium did not lead to significant shelf life extension. In conclusion, Lactobacillus reuteri R29 and its PIA-enriched cfs were found to be very promising alternatives for food bio-preservation. (C) 2017 Elsevier Ltd. All rights reserved.
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