期刊
FOOD CONTROL
卷 87, 期 -, 页码 100-108出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2017.12.002
关键词
Aflatoxins; Dual-wavelength fluorescence polarization immunoassay; Maize; Multi-mycotoxin detection; Zearalenones
资金
- Natural Science Foundation of China [31622057]
- National Science Foundation for Young Scientists of China [31502126]
To increase information content per screen, a dual-wavelength, homologous and high-throughput fluorescence polarization immunoassay (DWFPIA) for simultaneous detection of total aflatoxins (AFs) and family zearalenones (ZENs) was developed. Aflatoxin B-1 (AFB(1)) and zearalanone (ZAN) were labeled with different fluoresceins, and then combined with corresponding broad-specific antibodies to develop the DWFPIA. Under optimal conditions, the half maximal inhibitory concentrations of the FPIA in buffer were 2.68 mu g/L and 4.08 mu g/L for total four AFs (the weight ratio of AFB(1): Aflatoxin B-2: Aflatoxin G(1): Aflatoxin G(2) was 1: 1: 1: 1) and six ZEN analogs (the weight ratio of ZEN: ZAN: alpha-zearalenone: beta-zearalenone: alpha-zearalanone: beta-zearalanone was 1: 1: 1: 1: 1: 1) by mixing equal volumes (1: 1, v/v), respectively. The corresponding limit of detection values in maize flour samples were 4.98 mu g/kg and 11.03 mu g/kg, and the mean recoveries ranged from 78.6 to 103.6% with the coefficients of variation below 19.2%. The whole detection procedure, including sample preparation, took less than 30 min. Finally, the DWFPIA was applied to screen naturally contaminated maize flour samples, and the detection data were similar to those with HPLC-MS/MS. (C)2017 Elsevier Ltd. All rights reserved.
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