期刊
FOOD CONTROL
卷 89, 期 -, 页码 142-149出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2018.01.026
关键词
LCI; Antibacterial; S. aureus; Thermostability; Food industry
资金
- National Key Basic Research Program of China [2015CB755903]
- COMRA program [DY135-B-01]
- Fujian Regional Demonstration Project of Marine Economy Innovation and Development [2014FJPT10]
- Yantai Marine Economic Innovation and Development Demonstration City Industrial Chain Collaborative Innovation Project
- Natural Science Foundation of Fujian Province, China [2016J05079]
A novel LCI like antibacterial protein produced by Bacillus sp. MD-5, which was isolated from the Arctic Ocean sediment sample during the sixth Chinese National Arctic Expedition in 2014, was purified and characterized. After saturation of Bacillus sp. MD-5 culture supernatant with ammonium sulfate and separation with ion-exchange chromatography and high performance liquid chromatography (HPLC), an antibacterial protein (MD) was purified. The MD protein was determined to be a novel LCI like antibacterial protein with molecular weight of about 7.0 kDa using liquid chromatograph-mass spectrometer (LC-MS) identification and the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). It displayed antibacterial activity against Staphylococcus aureus at minimum inhibitory concentration (MIC) of 7.33 mu g/mL MD protein possessed high thermostability and wide range of pH tolerance, and had a certain extent of protease, surfactant and metal ion resistance. MD protein was suggested to inhibit the growth of S. aureus through inhibiting peptidoglycan biosynthesis in the view of TEM detection. These results suggested that MD may represent a novel antibacterial protein with potential application in controlling S. aureus in food industry. (C) 2018 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据