期刊
FOOD CHEMISTRY
卷 252, 期 -, 页码 271-276出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.01.087
关键词
Molecular mass; Pectin; Response Surface Methodology; Light scattering; Refraction index
资金
- MINECO of Spain [AGL2014-53445-R]
- Comunidad de Madrid [ALIBIRD-CM S-2013/ABI-272]
A high-performance size-exclusion chromatography (HPSEC) method coupled to Evaporative Light Scattering (ELS) and Refractive Index (RI) detectors were evaluated and compared for the molecular mass (Mw) estimation of pectin in a wide range (0.342-805 kDa). Instrumental parameters of the ELSD were optimised by Response Surface Methodology (RSM) being 73 degrees C the evaporator temperature and 0.9 mL/min the air flow rate. The linear range for the ELSD concentration response was wider (10-2250 mg/L) and better (R-2=0.985) than RID (10-1500 mg/L; R-2=0.875). The limits of detection (LOD) and quantitation (LOQ) for all pullulans hardly changed in ELSD (LOD: 1.22-1.99 mg/L; LOQ: 4.07-6.63 mg/L); however, RID showed huge variations (LOD: 0.49-10.41 mg/L; LOQ: 1.64-34.70 mg/L), which increased with the Mw. In general, responses of both detectors were similar for the Mw estimation, although pectin characterisation with HPSEC-ELSD exhibited better results in the lowest Mw compounds.
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