期刊
FOOD CHEMISTRY
卷 242, 期 -, 页码 75-82出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.09.035
关键词
Germination; Soybean; Digestion; Peptides; Anti-inflammatory; Antiproliferative
资金
- Ministry of Economy and Competitiveness (MINECO, Spain) [AGL2013-43247-R, AGL2015-66886-R]
- EU (FEDER)
- SEP-CONACyT [242860]
- Instituto Politecnico Nacional
- scholarship (CONACyT) [291062]
The aim was to investigate the potential of germinated soybean proteins as a source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions > 10, 5-10, and < 5 kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5-10 kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of beta-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.
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