期刊
FOOD CHEMISTRY
卷 261, 期 -, 页码 131-138出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.04.016
关键词
Lateral flow assay; Magnetite nanoparticles; Signal amplification; Furazolidone
资金
- National Natural Science Foundation of China [21675127, 31501560]
- New Century Excellent Talents in University [NCET-13-0483]
- Fundamental Research Funds for the Northwest A&F University of China [2014YB093, 2452015257]
- Development Project of Qinghai Key Laboratory [2017-ZJ-Y10]
We presented a signal amplified lateral flow assay (LFA) based on magnetite nanoparticles (MNPs) labeled dual-probe and applied it in the high sensitive and rapid on-site detection of furazolidone metabolite of 3-amino-2-oxazolidinone (AOZ). The amplified signal benefited from high affinity between two probes of MNPs labeled murine monoclonal antibody (MNPs-MAb) and goat anti-mouse antibody (MNPs-GAMA) and was achieved by the generation of dual-probe network complex. This developed method could realize high sensitive detection of AOZ with a threshold value of 0.88 ng mL(-1) and a detection limit of 0.044 ng mL(-1), the sensitivity was at least 10-fold improved than that of the traditional gold nanoparticle based LFA. This facile developed assay was successfully applied for rapid detection of AOZ in milk samples. The proposed method paves a new way for on-site screening of other hazardous substances in food and can be referred in all lateral flow assays.
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