期刊
FOOD CHEMISTRY
卷 262, 期 -, 页码 48-55出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.04.085
关键词
Immunochromatographic assay; Clenbuterol; Bacteria; Gold nanoparticles; Signal amplification
资金
- National Natural Science Foundation of China [21675127, 31501560]
- New Century Excellent Talents in University [NCET-13-0483]
- Fundamental Research Funds for the Northwest A&F University of China [2014YB093, 2452015257]
Immunochromatographic assays (ICAs) are most frequently used for on-site rapid screening of clenbuterol. To improve sensitivity, a novel probe with bacteria as signal carriers was developed. Bacteria can load a great deal of gold nanoparticles (AuNPs) on their surface, meaning much fewer antibodies are needed to produce clearly visible results, although low concentrations of antibody could also trigger fierce competition between free analyte and the immobilized antigen. Thus, a limited number of antibodies was key to significantly improved sensitivity. Analytical conditions, including bacterial species, coupling method, and concentration, were optimized. The visual detection limit (VDL) for clenbuterol was 0.1 ng/mL, a 20-fold improvement in sensitivity compared with traditional strips. This work has opened up a new route for signal amplification and improved performance of ICAs. Furthermore, inactivated bacteria could also be environment-friendly and robust signal carriers for other biosensors.
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