期刊
FOOD CHEMISTRY
卷 266, 期 -, 页码 343-349出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.06.030
关键词
HILIC-Quadrupole-Orbitrap-UHRMS; Nucleotides; Nucleosides; Nucleobases; White tea; Withering process
资金
- National Natural Science Foundation of China [31270735]
- Collaborative Innovation Center of Chinese Oolong tea Industry [2013-51]
- Fujian Agriculture and Forestry University
- special fund for Science and Technology innovation from Fujian Agriculture and Forestry University [CXZX2016117CXZX2017313]
- Faculty Postdoctoral Fellowship from Fujian Agriculture and Forestry University
Nucleotides, nucleosides, and nucleobases are important bioactive compounds. Recent studies suggested that they possess taste activity. However, it remains unknown about their presence in white tea and how they change during white tea manufacture. Here, we first established method based on hydrophilic interaction liquid chromatography coupled with quadrupole-orbitrap ultra high resolution mass spectrometry (HILIC-Quadrupole-Orbitrap-UHRMS) platform, then applied it to study the dynamic changes of nucleotides, nucleosides, and nucleobases during white tea withering process. Five compounds, including adenosine 5'-monophosphate monohydrate (AMP), guanosine 5'-monophosphate disodium salt hydrate (GMP), adenosine, cytidine, thymine and uracil, were detected from withering samples. They showed a general decline trend during white tea withering process, however, considerable amount of them was retained after withering for 48 h except adenosine which was below detection limit after withering for 21 h. This study provided a complete picture about nucleotides, nucleosides and nucleobases changes during white tea withering process.
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