期刊
FOOD CHEMISTRY
卷 255, 期 -, 页码 120-131出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.02.050
关键词
Red sweet pepper; NMR metabolite profile; Mass spectrometry; HPLC; Biogenic amines; Mycotoxins; alpha-Amylase inhibition
资金
- e-ALIERB Project [Regione Lazio - Dipartimento di Chimica e Tecnologie del Farmaco]
- Enrico and Enrica Sovena Foundation
A multi-methodological approach was applied to study red sweet peppers (Capsicum annuum L.) ecotype Cornetto di Pontecorvo grown in a greenhouse or in open field. This approach includes morphological analysis, chemical composition determination, and biological activity evaluation of different extracts from pepper fruits. Untargeted analyses, namely NMR spectroscopy and mass spectrometry, allowed the comprehensive pepper metabolite profile of pepper pulp, peel and seeds hydroalcoholic and organic extracts to be determined, showing the presence of sugars, organic acids, amino acids and other secondary metabolites. Targeted analyses, such as HPLC-PDA, HPLC-TLC and spectrophotometric analyses allowed polyphenols, tannins, flavonoids and pigments content to be determined. Samples quality and freshness were verified by the low content of biogenic amines and mycotoxins, as determined using HPLC-FLD and HPLC-MS, respectively. Preliminary biological results demonstrated the ability of the organic extracts to inhibit alpha-amylase, a key enzyme in the control of glucose metabolism.
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