期刊
FOOD CHEMISTRY
卷 251, 期 -, 页码 103-109出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.12.090
关键词
Garlic; Allium sativum L.; Transcription profiling; Normalisation; Alliinase; Cysteine synthase; Housekeeping genes
资金
- National Agency for Agriculture Research of the Czech Republic [QJ1210158]
- Minsitry of Agriculture of the Czech Republic [RO0416]
Garlic is a valuable source of healthy compounds, including secondary metabolites rich in sulphur such as cysteine sulphoxides (CSOs). Here, we present new qRT-PCR assays analysing the transcription of two genes encoding key enzymes in CSO biosynthetic pathways (cysteine synthase and alliinase) in developing garlic. We also identified a set of genes (ACT I, GAPDH, and TUB) to use as transcription normalisation controls. We showed that the (normalised) transcription of both enzymes was highest during sprouting and decreased significantly in fully developed leaves, which are the major CSO-producing organs. Transcriptional activity further declined at the end of the growing season. Different cultivars show similar sulphur metabolism gene expression when European garlics were compared to Chinese and American genotypes. The qRT-PCR assays presented are also suitable for investigating the effects of agricultural practices on CSO formation in garlic to satisfy consumer demands.
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