Alpha-linolenic acid-loaded oil/water microemulsion: Effects of phase behaviour simulation and environmental stress on phase stabilizing and anti-oxidation capacity

alpha-Linolenic acid (ALA)-loaded microemulsion (ME) was prepared from isoamyl acetate, polyoxyethylene ether 35 (EL-35), ethanol and water. The dynamic phase behaviour was simulated using dissipative particle dynamics (DPD), which showed that spherical ME was formed at water/oil ratios of 1:9 and 9:1, while a lamellar structure with distinctive water-course and oil layer appeared at ratios of 3:7, 5:5, and 7:3. Phase stabilizing and anti-oxidation effect of environmental stresses on ALA-loaded microemulsion were investigated. Results showed that the ME region was large and had good environmental tolerance. Subsequently, the investigation of anti-oxidation stability revealed that more than 60% ALA of ALA-loaded ME could be protected from oxidation under environmental stresses. Furthermore, ALA-loaded ME was applied in aqueous-based foods. The transparency, precipitate, stratification and phase separation were used to evaluate influence of ME on product properties, confirming great feasibility and stability of ALA-loaded ME for practical applications.