期刊
FOOD CHEMISTRY
卷 243, 期 -, 页码 74-81出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.09.109
关键词
Interaction; Cinnamaldehyde; Cinnamic acid; Human serum albumin; Saturation transfer difference nuclear magnetic; resonance; Interference
资金
- Large-scale Science Instrument Shareable Platform Construction of Sichuan Province [2015JCPT0005-15010102]
- Large-scientific Instruments Sharing Platform Ability Construction of Sichuan Province [2016KJTS0037]
Considering the adverse effect of food additives on humans, thorough research of their physiological effects at the molecular level is important. The interactions of cinnamaldehyde (CNMA), a food perfume, and its major metabolite cinnamic acid (CA) with human serum albumin (HSA) were examined by multiple-spectroscopies. NMR analysis revealed CNMA and CA both bound to HSA, and STD-NMR experiments established CNMA and CA primarily interacted with site I and site II of HSA, respectively. The ligands caused strong quenching of HSA fluorescence through a static quenching mechanism, with hydrophobic and electrostatic interaction between CNMA/CA and HSA, respectively. UV-vis absorption and CD results showed ligands induced secondary structure changes of HSA. Binding configurations were proved by docking method. Furthermore, binding constants of CNMA/CA-HSA systems were influenced by the addition of four other food additives. These studies have increased our knowledge regarding the safety and biological action of CNMA and CA.
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