期刊
FOOD CHEMISTRY
卷 240, 期 -, 页码 391-395出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.07.153
关键词
Oat; ELISA; Gluten; Kernel-based gluten contamination, gluten analysis; Gluten-free, serving size
资金
- PepsiCo Inc.
- Quaker Foods and Snacks (QFS)
A step-wise, 'test-all-positive-gluten' analytical methodology has been developed and verified to assess kernel-based gluten contamination (i.e., wheat, barley and rye kernels) during gluten-free (GF) oat production. It targets GF claim compliance at the serving-size level (of a pouch or approximately 40-50 g). Oat groats are collected from GF oat production following a robust attribute-based sampling plan then split into 75-g sub-samples, and ground. R-Biopharm R5 sandwich ELISA R7001 is used for analysis of all the first 15-g portions of the ground sample. A > 20-ppm result disqualifies the production lot, while a > 5 to < 20-ppm result triggers complete analysis of the remaining 60-g of ground sample, analyzed in 15-g portions. If all five 15-g test results are < 20 ppm, and their average is < 10.67 ppm (since a 20-ppm contaminant in 40 g of oats would dilute to 10.67 ppm in 75-g), the lot is passed.
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