Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate

Measuring reducing sugar is a common practice in carbohydrate research, and the colorimetric assay developed by Somogyi and Nelson has a high sensitivity in a broad concentration range. However, the method is time-consuming when analyzing a large number of samples. In this study, a modified Somogyi-Nelson assay with excellent accuracy and sensitivity was developed using a 96-well microplate. This microassay greatly improves the analytic capacity and efficacy of the method. (C) 2017 Published by Elsevier Ltd.