期刊
FOOD CHEMISTRY
卷 239, 期 -, 页码 276-286出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.06.117
关键词
Immobilization; Pullulanase; Resistant starch; Hydrolysis; Stability
资金
- National Natural Science Foundation of China [31601413]
- Natural Science Foundation of Jiangsu Province [BK20160168]
- Postdoctoral Research Funding Plan of China [2016M600364]
In this study, pullulanase was firstly immobilized by covalent bonding onto chitosan/Fe3O4 nanoparticles or encapsulation in sol-gel after bonding onto chitosan/Fe3O4 nanoparticles, and then the immobilized pullulanase was used for the effective production of resistant starch (RS). The highest RS content (35.1%) was obtained under the optimized condition of pH 4.4, enzyme concentration of 10 ASPU/g and hydrolysis time of 12 h when debranched by free pullulsanase, indicating that RS content was significantly (p < 0.05) increased when compared to native starch (4.3%) and autoclaved starch (12.5%). Under these conditions, the immobilized pullulanase (10 ASPU/g dry starch) yielded higher RS content compared to free enzyme (10 ASPU/g dry starch), especially, the pullulanse immobilized by sol-gel encapsulation yielded the highest RS content (43.4%). Moreover, compared to starches hydrolyzed by free pullulanase, starches hydrolyzed by immobilized pullulanase showed a different saccharide profile of starch hydrolysate, including a stronger peak C (MW = 5.0 x 10(3)), as well as exhibited an additional absorption peak around 140 degrees C. Reusability results demonstrated that pullulanase immobilized by sol-gel encapsulation had the advantages of producing higher RS content as well as better operational stability compared to pullulanase immobilized by cross-linking. The resulting enhanced RS content generated by the process described in this work could be used as an adjunct in food processing industries. (C) 2017 Elsevier Ltd. All rights reserved.
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