期刊
FOOD CHEMISTRY
卷 259, 期 -, 页码 25-30出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.03.099
关键词
Bioactive peptides; Egg white proteins; Adipogenic peptides; Liquid chromatography
资金
- Alberta Livestock and Meat Agency (ALMA)
- Egg Farmers of Canada
- Egg Farmers of Alberta
- Natural Sciences and Engineering Research Council (NSERC) of Canada
Egg proteins are a good source of bioactive peptides. Previous work from our research group has demonstrated the potential of egg white hydrolysate (EWH) for enhancing adipogenic differentiation and insulin signalling in 3T3-F442A pre-adipocytes. EWH was prepared by a combination of thermolysin and pepsin. Here, in this study, we aimed to identify the responsible peptide(s) in EWH. EWH was fractionated stepwise by ultrafiltration, C18 Sep-Pack cartridge, cation-exchange chromatography, and reverse-phase chromatography. The two most active fractions were analyzed by LC-MS/MS and 42 peptides were identified. Eleven peptides were synthesized and WEKAFKDED, QAMPFRVTEQE, ERYPIL, and VFKGL from ovalbumin were validated with peroxisome pro-liferator-associated receptor gamma stimulatory activity in adipocytes. For the first time, adipogenic differentiating peptides were characterized from egg white proteins. This data is pivotal for future structure-function studies of adipogenic peptides.
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