期刊
FOOD CHEMISTRY
卷 263, 期 -, 页码 29-36出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.04.092
关键词
Galactooligosaccharides; GOS; beta-galactosidase; High-performance anion-exchange; chromatography; k-factor
资金
- project of National Standard of PR China (Determination of Galactooligosaccharides (GOS) in Foods) - National Health Commission of People's Republic of China [spaq-2012-74]
This study describes a method for the simultaneous determination of galactose, glucose, lactose and galactooligosaccharides (GOS) in GOS raw materials (GOS syrups and powdered GOS) by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The GOS raw materials were extracted with phosphate buffer. The extract was then treated with beta-galactosidase to hydrolyze GOS and lactose. The total amounts of galactose and glucose released from GOS and lactose were determined in the treated solution. Free galactose, glucose and lactose were determined in the initial solution. The glucose in a beta-galactosidase solution was also determined. The content of GOS in GOS raw materials was calculated by the increment of galactose and glucose after GOS were hydrolyzed, and the glucose and galactose also released from lactose were taken into consideration. The validated method has been successfully applied to determine the content of galactose, glucose, lactose and GOS in GOS syrups and powdered GOS.
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