期刊
FOOD ANALYTICAL METHODS
卷 11, 期 10, 页码 2865-2884出版社
SPRINGER
DOI: 10.1007/s12161-018-1244-8
关键词
Veterinary drugs; Multi-residue analysis; Meat; Pass-through cleanup; UPLC-MS/MS
资金
- National Key Research and Development Program of China [2017YFC1601600]
A quantitative method using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of 60 compounds, belonging to a variety of veterinary drug (VD) classes, in meat. The included analytes belong to the following VD classes: beta-agonists, sulfanilamides, quinolones, macrolides, tetracyclines, beta-lactams, nitroimidazoles, glucocorticoids, sex hormones, chloromycetins, sedatives, and olaquindox metabolite. The effective PRiME pass-through cleanup procedure was used to ensure high extraction efficiency and good sample cleanup after a simple liquid extraction of the meat samples with acetonitrile/water. The developed method was validated successfully. Mean recoveries for all analytes ranged from 80 to 116%, with the relative standard deviations (RSDs) <= 7.8%. Limits of quantification were in the range 0.05-3.0 mu g kg(-1) and limits of detection were in the range 0.1-10 mu g kg(-1). The matrix effect was evaluated for the different meat matrices and was found to be markedly different in different matrices. The validated method was used in a pilot study to analyze real samples of pork, beef, mutton, chicken, and pork liver, lambs' liver, and chicken liver. Trace amounts of beta-agonists, oxytetracycline, quinolones, chloromycetin sulfadimidine, and 3-methyl-quinoxaline-2-carboxylicacid were detected in these samples. In conclusion, this workflow can provide a simpler and more cost-effective alternative to conventional analytical methods and is compatible with processing large sample numbers in a short time period.
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