Isothermal Amplification Technologies for the Detection of Foodborne Pathogens

Isothermal amplification technology has developed rapidly in recent years. This form of nucleic acid amplification technology has the characteristics of high specificity, high sensitivity, convenience, and low cost. Isothermal amplification detection is expected to become an especially important method for the detection of foodborne and environmental pathogens. This review highlights the reaction principles, applications, advantages, and limitations of various isothermal amplification methods that have been used for the sensitive detection of nucleic acids of foodborne pathogens, including loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), strand displacement amplification (SDA), cross-priming amplification (CPA), nucleic acid sequence-based amplification (NASBA), and recombinase polymerase amplification (RPA). Most of these isothermal amplification methods have great potential for practical application to the detection of foodborne pathogens.