期刊
FISH & SHELLFISH IMMUNOLOGY
卷 73, 期 -, 页码 197-206出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.12.020
关键词
Trachinotus ovatus; Dandelion extracts; Intestinal morphology; Intestinal immunity; Expression of tight junction proteins
资金
- Special Scientific Research Funds for public service industry (Agriculture) [201403011]
- Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences [2014ZD02]
- Guangdong Provincial Oceanic Fisheries Science and Technology Project [A201601C04]
- Guangdong Provincial Natural Science Foundation [2016A030313447]
Intestinal morphology, antioxidant status, immune function and tight junction proteins mRNA expression were examined in golden pompano (Trachinotus ovatus) that fed respectively six diets containing dandelion extracts (DE) at 0, 0.5, 1, 2, 4 and 10 g kg(-1) after 8 weeks feeding. The study indicated that dietary DE significantly improved intestinal antioxidant abilities by increasing SOD, CAT, T-AOC activities and up-regulating intestinal cat, gpx mRNA levels, but by decreasing MDA content and down-regulating intestinal keapl mRNA levels in golden pompano. Meanwhile, dietary DE improved intestinal morphology, suggesting that enhances intestinal digestion and absorption, by increasing muscle thickness, villus length, villus width and villus number in the foregut and hindgut; as well as villus number, villus width and muscle thickness in the midgut (P<.05). Dietary DE enhanced intestinal barrier function by increasing intestinal zo-1 and occludin mRNA levels, but by decreasing the mRNA levels of claudin-12 and claudin-15. Furthermore, dietary DE improved intestinal immunity via increasing goblet cells numbers and regulating expression of immune-related genes. In conclusion, dietary DE supplementation promoted intestine health by improving intestine morphology, immunity, antioxidant abilities and intestinal barrier in golden pompano.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据