期刊
FISH & SHELLFISH IMMUNOLOGY
卷 74, 期 -, 页码 242-249出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2017.12.029
关键词
Antimicrobial peptide; Defensin; Innate immunity; Mollusks; Mussels; Perna viridis
资金
- National Natural Science Foundation of China [31470431, 31100262, 31470389]
- Guangdong Natural Science Foundation [2014A030308017, 2016A030313052]
- DEGP [2015KTSCX125]
- Shenzhen special funds for Bio-industry development [NYSW20140327010012]
- Shenzhen Grant Plan for Science Technology [JCYJ20160422171614147]
- SRF [[2012]940]
Defensin is one of the most diversified groups of antimicrobial peptides in invertebrate. In the present study, a novel defensin member referred as Pv-Def was identified and characterized from Asian green mussel Perna viridis. Using in silico survey of several EST databases released from diverse tissues of P. viridis, a single peptide referred as Pv-Def was predicted as defensin homologue with Mytilus counterparts. Further analysis on gene structure revealed that Pv-Def was 1001 nt in length and consisted of 3 exons and 2 introns. The precursor of Pv-Def was composed of a signal peptide of 19 amino acids and a mature peptide of 45 amino acids. The mature PvDef peptide contains 6 cysteines which formed 3 disulfide bonds at C-27(1)- C-54(4), C-40(2)- C-60(5) and C-44(3)- C-62(6). Like most of the defensin family members, mature Pv-Def peptide included an alpha helix and 2 beta strands. Pv-Def showed significantly tissue-specific expression pattern, while highest transcription level was observed in hepatopancreas, which was about 900 folds to that in hemocytes. Moreover, the expression of Pv-Def mRNA in hemocytes was significantly and accurately up-regulated at different time intervals by Vibrio parahaemolyticus challenge. Interestingly, phylogenetic analysis suggested that the Pv-Def possesses closest relationships with arthropods counterparts rather than other mollusk defensins. To our knowledge, this is the first time that a defensin member was reported in Asian green mussel P. viridis.
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