期刊
FASEB JOURNAL
卷 32, 期 8, 页码 4600-4611出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.201701462RR
关键词
surface tension; purinergic signaling; P2Y(2); calcium; lung surfactant
资金
- Spanish Ministry of Economy and Competitiveness [BIO2012-30733, BIO2015-67930-R, EEBB-I-2015-10363]
- Regional Government of Madrid [P2013/MIT-2807]
- Deutsche Forschungsgemeinschaft (DFG) [DI1402/3-1]
- Ministry of Science, Research, and the Arts of Baden-Wurttemberg [Az: 32-7533.-6-10/15/5]
- DFG [SFB 1149/1, A05]
- Formacion de Personal Investigador (FPI) Fellowship from the Spanish Ministry of Economy and Competitiveness
The release of pulmonary surfactant by alveolar type II (ATII) cells is essential for lowering surface tension at the respiratory air-liquid interface, stabilizing the lungs against physical forces tending to alveolar collapse. Hydrophobic surfactant protein (SP)-B ensures the proper packing of newly synthesized surfactant particles, promotes the formation of the surface active film at the alveolar air-liquid interface and maintains its proper structure along the respiratory dynamics. We report that membrane-associated SP-B efficiently induces secretion of pulmonary surfactant by ATII cells, at the same level as potent secretagogues such as ATP. The presence in the extracellular medium of lipid-protein complexes containing SP-B activates the P2Y(2) purinergic signaling pathway that ultimately triggers exocytosis of lamellar bodies by ATII cells. Our data suggest that SP-B prompts Ca2+-dependent surfactant secretion via ATP release from ATII cells. This result implies that SP-B is not only an essential component for the biophysical function of surfactant but is also a central element in the alveolar homeostasis by eliciting autocrine and paracrine cell stimulation.Martinez-Calle, M., Olmeda, B., Dietl, P., Frick, M., Perez-Gil, J. Pulmonary surfactant protein SP-B promotes exocytosis of lamellar bodies in alveolar type II cells.
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