期刊
FUNGAL GENETICS AND BIOLOGY
卷 81, 期 -, 页码 142-149出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2014.10.018
关键词
Metarhizium robertsii; Polyketide synthase; Pigment; Melanin; Virulence; Stress response
资金
- National Nature Science Foundation of China [31225023]
- Strategic Priority Research Program of Chinese Academy of Sciences [XDB11030100]
Fungal polyketide synthases (PKSs) and their related gene clusters are highly diversified at both interand intra-specific levels. The most well characterized PKS enzymes include those responsible for the biosynthesis of polyketide pigments such as melanins. The genome of the insect pathogenic fungus Metarhizium robertsii contains 20 type I PKSs but none has been functionally characterized. In this study, two PKS genes (designated as MrPks1 and MrPKs2) showing homologies to those counterparts for the biosynthesis of heptaketide pigments and dihydroxynaphthalene (DHN)-melanins, respectively, were deleted in two different strains of M. robertsii. The results indicated that disruption of MrPks1 but not MrPks2 impaired fungal culture pigmentation and cell wall structure. In addition to the negative effect of the DHN-melanin pathway inhibitor, it was postulated that DHN-melanin would not be produced by M. robertsii. Various assays revealed that the stress resistance abilities against ultraviolet radiation, heat shock and oxidants, as well as virulence against insects were not impaired in Delta MrPks1 and Delta MrPks2 isolates when compared with the wild-type strain. Thus, the non-melanin pigment(s) produced by the fungus do not contribute to cell damage protection and pathogenicity in M. robertsii. Physiological differences were evident in the two examined wild-type strains. The results from this study advance the understanding of functional divergence of fungal PKSs. (C) 2014 Elsevier Inc. All rights reserved.
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