期刊
BIOSENSORS-BASEL
卷 5, 期 2, 页码 337-363出版社
MDPI
DOI: 10.3390/bios5020337
关键词
molecular probes; sensors; metal ions; proteins; DNA
资金
- Grants-in-Aid for Scientific Research [15K06592] Funding Source: KAKEN
This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio) molecular recognition based on these fluorescence phenomena, various fluorescent elements such as small organic molecules, enzymes, antibodies, and oligonucleotides have been designed and synthesized over the past decades. This review describes the immense variety of fluorescent probes that have been designed for the recognitions of ions, small and large molecules, and their biological applications in terms of intracellular fluorescent imaging techniques.
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