期刊
EXPERIMENTAL DERMATOLOGY
卷 27, 期 5, 页码 473-475出版社
WILEY
DOI: 10.1111/exd.13483
关键词
epithelium; steroids; three-dimensional tissue models; toxicity; xenobiotic metabolism
类别
资金
- National Centre for the Replacement, Refinement & Reduction of Animals in Research (NC3R) UK CRACK-IT Challenge 20 award
- MRC [MR/L006758/1] Funding Source: UKRI
CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue-engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl-O-methyl-cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time-dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据