期刊
MOLECULAR BIOTECHNOLOGY
卷 57, 期 6, 页码 489-498出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-015-9842-9
关键词
Aeluropus lagopoides; HKT transporter; Salt stress; AlHKT2;1; S. cerevisiae
资金
- CSIR [OLP 0067]
- CSIR
The high-affinity potassium transporters (HKT) are highly important for stress tolerance in plants as they uniquely maintain K+/Na+ ratio for their survival and growth. In this study a novel HKT gene AlHKT2;1 was isolated and characterized from salt secreting halophyte, Aeluropus lagopoides. The AlHKT2;1 cDNA comprised of an open reading frame of 1,581 bp, encoding a protein of 526 amino acid residues. It belongs to class II HKTs and showed high homology with other HKT genes. Functional characterization of AlHKT2;1 in both K+ uptakeaEurodeficient (W Delta 6) and Na+-sensitive yeast mutants (G19) showed the characteristic feature of low-affinity K+ transporter supporting the growth at > 1 mM KCl concentration. The transformed yeast cells showed high sensitivity to NaCl; however, the addition of KCl along with NaCl support the growth of AlHKT2;1 expressing mutant. Ion content analysis of yeast cells with AlHKT2;1 grown in high NaCl medium supplemented with KCl revealed that salt tolerance was correlated with accumulation of K+ during salt stress. These results suggest that AlHKT2;1 plays an important role in the K+ uptake during salt stress and in maintaining a high K+/Na+ ratio in the cytosol.
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