期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 818, 期 -, 页码 184-190出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2017.10.050
关键词
l-Theanine; ETEC; Hepatic injury; Apoptosis response
资金
- National Key Research and Development Program of China [2017YFD0400803]
- Science and Technology Major Project of Hunan Province, China [2017NK1020]
L-Theanine (LTA; gamma-glutamylethylamide), a peculiar non-protein-derived amino acid isolated from tea, is widely used as a functional ingredient and dietary supplement. L-Theanine has been confirmed to have hepatoprotective effects, but the underlying mechanism remains unknown. This study investigated the protective effect of L-Theanine-in vivo, using an enterotoxigenic Escherichia coli (ETEC)-infected mouse model. L-Theanine significantly decreased the elevated serum activities of both aspartate aminotransferase (AST) and alanine aminotransferase (ALT), two biomarkers of hepatic impairment. This was consistent with histopathological images from the microscopic observation of liver tissue. In addition, L-theanine significantly increased the mRNA and protein expression of Bcl-2 and decreased the expression of Bax, anti-and pro-apoptotic molecules, respectively, compared with levels in the ETEC control group. The expression of cleaved caspase-3 protein in the group pre-treated with L-theanine was significantly lower than that in the ETEC group. Additionally, decreases in extracellular signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase(JNK1/2) MAPK phosphorylation were observed in the L-theanine pre-treated group. Our study demonstrates that L-theanine possesses anti-apoptotic activity, which can be attributed to suppression of the intrinsic mitochondria-mediated apoptosis and MAPK phosphorylation signaling pathways.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据