期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 826, 期 -, 页码 133-139出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2018.02.044
关键词
Oridonin; Endothelial cells; Vascular inflammation
资金
- National Natural Science Foundation of China (Less Developed regions) [81560817]
- Guizhou Provinceial Science and Technology Funds, China [[2013]2074]
- Research Foundation for Young Scholars of Education Bureau of guizhou Province [[2015]438]
- State Key Program for Basic Research of Guizhou Province [[2015]2002]
Oridonin, an active diterpenoid compound isolated from the plant Rabdosia Rrubescens, has various pharmacological activities, including antioxidant, anti-tumor capacities and anti-inflammation. In the present study, we explore the role of oridonin in regulating endothelial inflammation and its underlying mechanism. Endothelial cell-monocyte interaction was detected by Leukocyte-endothelium Adhesion Assay. The protein expression was measured by Western blot. NF-kappa B p65 translocation was measured by immunofluorescence. Acute lung inflammation model was used to evaluate leukocyte infiltration in vivo. The endothelial-leukocyte adhesion and the leukocyte transmigration were profoundly reduced by oridonin. Oridonin dramatically inhibited the expression of TNF-alpha-induced endothelial adhesion molecules (intercellular adhesion molecule-1 (ICAM-1); vascular cell adhesion molecule-1 (VCAM-1) and E-selectin) and the pro-inflammatory cytokine (IL-6, IL-8 and monocyte chemoattractant protein-1(MCP-1)). Oridonin suppressed the penetration of the leukocyte in the acute lung injury mice model. Furthermore, Oridonin also suppressed the TNF-alpha-activated MAPK and Nuclear factor kappa B (NF-kappa B) activation. Our results suggest that oridonin has the anti-inflammatory properties in endothelial cells, at least in part, through the suppression of MAPK and NF-kappa B activation, which may have a potential therapeutic use for inflammatory vascular diseases.
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